If you pull one of the wires and nothing changes, then that cylinder is dead (so to speak).
Could be a bad wire, bad plug, bad injector, bad injector wiring or possible a problem with that cylinder engine wise like extremely low or no compression..... I would first check the resistance of that particular wire (Typically, OEM style wires are 1000ohms per foot. High performance wires are usually a bit lower (resistance value per foot))... Another way to check is to grab an extra plug and pull that wire out (off of cylinder), plug in the exta plug that you grabbed, lay the plug thread / hex area on a good grounded surface and start the car. If your getting good spark then that rules out the plug wire. Next pull the plug and inspect it, or simply try another plug. If no luck with that, then the problem is either fuel related like bad injector or wiring or a problem with that cylinder in the engine.